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KMID : 0545120160260020255
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 2 p.255 ~ p.262
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Purification and Characterization of a New κ-Carrageenase from the Marine Bacterium Vibrio sp. NJ-2
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Zhu Ben Wei
Ning Li Min
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Abstract
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The carrageenan-degrading marine bacterium Vibrio sp. strain NJ-2 was isolated from rotten red algae, and κ-carrageenase with high activity was purified from the culture supernatant. The purified enzyme with molecular mass of 33 kDa showed the maximal activity of 937 U/mg at 40°C and pH 8.0. It maintained 80% of total activity below 40°C and between pH 6.0 and 10.0. The kinetics experiment showed the Km and Vmax values were 2.54 mg/ml and 138.89 mmol/min/mg, respectively. The thin layer chromatography and ESI-MS analysis of hydrolysates indicated that the enzyme can endolytically depolymerize the κ-carrageenan into oligosaccharides with degrees of depolymerization of 2-8. Owing to its high activity, it could be a valuable tool to produce κ-carrageenan oligosaccharides with various biological activities.
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KEYWORD
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κ-carrageenase, purification, characterization, oligosaccharides
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